Composite

Part:BBa_K3747015:Design

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-21)


pMMO-m1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 377
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 1043
  • 1000
    COMPATIBLE WITH RFC[1000]


pET-28a(+)-pMMO_m1.The particulate methane monooxygenase mimic plasmid as described in Kim et al 2019 [1]. This high copy number plasmid harbors the pMMO active site mimic by scaffolding the substructures pMMO-B1 BBa_K3747005 and pMMO-B2 BBa_K3747007 on a human heavy-chain ferritin (huHF) BBa_K3747008 scaffold. This is under control of a lac operator and T7 promotor.

Source

Synthetic, mimicking pMMO from Methylococcus capsulatus

References

[1] Kim, H.J., Huh, J., Kwon, Y.W. et al. Biological conversion of methane to methanol through genetic reassembly of native catalytic domains. Nat Catal 2, 342–353 (2019). https://doi.org/10.1038/s41929-019-0255-1